A Comparative Study of Materials Suitable for the Cultivation of Clostridia.
نویسنده
چکیده
Both plant and animal materials have been utilized in the cultivation of members of the Genus Clostridium, chiefly in the form of mashes and infusions (McClung and McCoy, 1934, and Castell, 1942). Thioglycollate broth, introduced by Brewer in 1940, was the first light, transparent medium capable of growing clostridia without anaerobic jars, which could be stored for weeks and used without preheating. This medium contains meat infusion and gives excellent results with aerobes and with pathogenic and proteolytic anaerobes, but grows the butyric group of clostridia irregularly and only when comparatively large inocula are used (McClung, 1940). This fact led to an investigation of readily available and inexpensive sources for basic media suitable for the cultivation of butyric-acid and butyl-alcohol-producing clostridia as well as of the more readily cultivable species. In 1943 Brewer described a method for digestion of vegetable materials with papain and he stated that, when used in thioglycollate media, such materials were capable of supporting the growth of most butyric organisms.
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 47 1 شماره
صفحات -
تاریخ انتشار 1944